LTEP-sm（人小細(xì)胞）對(duì)于每種細(xì)胞因子應(yīng)仔細(xì)閱讀說(shuō)明書，注意每批的細(xì)節(jié)。在使用細(xì)胞因子前，瞬時(shí)離心試劑瓶，盡可能多地收回其中的細(xì)胞因子。
LTEP-sm（人小細(xì)胞）來(lái)源可靠（源于ATCC、ECACC、DSMZ、JCRB等），活力>95%，貼壁性好。我們從試劑、包被器皿、凍存原代細(xì)胞、新鮮原代細(xì)胞、原代細(xì)胞的分子學(xué)實(shí)驗(yàn)等提供全程服務(wù)。我司擁有專業(yè)的實(shí)驗(yàn)室，可無(wú)菌操作并提供相關(guān)科研項(xiàng)目解決方案以及實(shí)驗(yàn)服務(wù)。
服務(wù)流程：
預(yù)約登記→辦理相關(guān)手續(xù)→復(fù)蘇細(xì)胞→細(xì)胞質(zhì)量檢查→發(fā)送細(xì)胞（或自己來(lái)取細(xì)胞）→細(xì)胞售后技術(shù)咨詢答疑。
養(yǎng)方法如下：
1、從手術(shù)室無(wú)菌取腦髓灰質(zhì)或白質(zhì)后，仔細(xì)剝除腦膜、血管和纖維成分，置Hanks液中漂洗一、二次。
2、置于30—50倍體積的Hanks液中，此時(shí)腦組織比較柔軟，反復(fù)吹打即制成細(xì)胞懸液。
3、把懸液注入離心管室溫中直立5—10分鐘后，細(xì)胞或細(xì)胞團(tuán)快自然下沉，脂肪等雜物易漂浮，可吸除上層、反復(fù)二、三次，即可排除脂肪成分和其它碎塊并獲較多細(xì)胞成分。
4、在沉降物中加入適量培養(yǎng)液，通過(guò)紗網(wǎng)成紗布過(guò)濾，計(jì)數(shù)并調(diào)整細(xì)胞密度。
5、接入培養(yǎng)瓶或皿中，置5%CO2溫箱中培養(yǎng)。
適應(yīng)環(huán)境過(guò)程較長(zhǎng)，接種后貼壁較慢。貼壁后短期內(nèi)也可能不見(jiàn)細(xì)胞分裂現(xiàn)象，然而一旦生長(zhǎng)后即能迅速進(jìn)入旺盛的增殖狀態(tài)。細(xì)胞傳代可以0.25%胰酶消化處理。
我司其他相關(guān)銷售產(chǎn)品：
Cs-(036)-007524    中國(guó)倉(cāng)鼠XB淋巴細(xì)胞株/細(xì)胞雜交瘤MR1[derivativeofHB-11048]細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007525    中國(guó)倉(cāng)鼠倉(cāng)鼠肺細(xì)胞株/細(xì)胞,R1610細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007526    中國(guó)倉(cāng)鼠倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞亞株,CHO-K1細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007527    中國(guó)倉(cāng)鼠二氫葉酸還原酶缺陷細(xì)胞株/細(xì)胞，CHO/dhFr-細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007528    中國(guó)倉(cāng)鼠肺細(xì)胞株/細(xì)胞，CHL/IU[CHL-11]細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007529    中國(guó)倉(cāng)鼠肺細(xì)胞株/細(xì)胞,CHL細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007530    中國(guó)倉(cāng)鼠肺細(xì)胞株/細(xì)胞，CHL細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007531    中國(guó)倉(cāng)鼠肺細(xì)胞株/細(xì)胞，V79-4細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007532    中國(guó)倉(cāng)鼠肺細(xì)胞株/細(xì)胞，V79細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007533    中國(guó)倉(cāng)鼠肺細(xì)胞株/細(xì)胞；CHL    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007534    中國(guó)倉(cāng)鼠肺細(xì)胞株/細(xì)胞；R 1610 [R1610]    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007535    中國(guó)倉(cāng)鼠肺細(xì)胞株/細(xì)胞；V79    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007536    中國(guó)倉(cāng)鼠肺細(xì)胞株/細(xì)胞CHL細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007537    中國(guó)倉(cāng)鼠肺細(xì)胞株/細(xì)胞R 1610 [R1610] 細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007538    中國(guó)倉(cāng)鼠肺細(xì)胞株/細(xì)胞V79細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007539    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007540    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007541    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（二氫葉酸還原型缺陷型）CHOdhFr細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007542    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（二氫葉酸還原型缺陷型）CHOdhFr-細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007543    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（APP-PS1(C410Y)雙基因修飾）7WCY1.0細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007544    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（APP-PS1(M146L)雙基因修飾）7WML6.0細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007545    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（APP-PS1雙基因修飾）7WPS1細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007546    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（APP基因修飾）7WD10細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007547    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。?； EPO-CHO-T    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007548    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。?；CHO 1beta9,12.5 Clone 36    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007549    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。?；CHO DUX B11 Carrying pBSCRLc/pTCSgpt clone 35.6    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007550    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。?；CHO Hu 164 SCF 17    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007551    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克隆）；CHO-5beta 41,2.5 CL5 Carrying P5b/dhfr    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007552    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。?；CHO-beta2(414) CL.32 pTGF-beta2(414)    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007553    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。?；CHO-DUKXB11-prepro-TGFβ1 cDNA    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007554    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克隆）；CHO-DUKXB11-prepro-TGFβ1 Cdna-TGF-β1 Dinding Protein cDNA    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007555    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。?；CHO-DUKXB11-prepro-TGFβ2 cDNA-TGF-β Dinding Protein cDNA    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007556    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。籆HO-DUKXB11-TGF-β Dinding Protein cDNA    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007557    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。籆HO-HCV-C    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007558    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。?；CHO-HCV-E1    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007559    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。?；CHO-HCV-E2    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007560    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。籆HO-HCV-NS2    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007561    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克隆）；CHO-HCV-NS3    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007562    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。?；CHO-HCV-NS4A    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007563    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。籆HO-HCV-NS4B    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007564    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克隆）；CHO-HCV-NS5A    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007565    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克隆）；CHO-HCV-p7    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007566    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。?；DUKXB11 carrying pDEF202-hTPO-163    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007567    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。籇UKXB11 carrying pDEF202-hTPO-P1    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007568    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。〤HO1beta9,12.5Clone36細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007569    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克隆）CHO-5beta41,2.5CL5CarryingP5bdhfr細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007570    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。〤HO-beta2(414)CL.32pTGF-beta2(414)細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007571    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。〤HO-DUKXB11carryingpDEF202-hTPO-163細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007572    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。〤HO-DUKXB11carryingpDEF202-hTPO-P1細(xì)胞株/細(xì)胞    ，規(guī)格：    >5×100000cells/瓶
Cs-(036)-007573    中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞（亞系克?。〤HO-DUKXB11-prepro-TGFβ1cDNA-TGF-β1DindingProt    ，規(guī)格：    >5×100000cells/瓶