更新時(shí)間:2024-06-11
我司F9(小鼠畸瘤細(xì)胞)的細(xì)胞培養(yǎng)基其組成成分主要有:水、氨基酸、維生素、碳水化合物、無(wú)機(jī)離子及其他一些入核酸降解物、激素等。保證運(yùn)輸中的正常生長(zhǎng),關(guān)于其價(jià)格、報(bào)價(jià)、貨期,已打造成抗體在華東地區(qū)Z大交易平臺(tái)之一,歡迎您的咨詢!
免費(fèi)咨詢:021-54720761
F9(小鼠畸瘤細(xì)胞)對(duì)于每種細(xì)胞因子應(yīng)仔細(xì)閱讀說(shuō)明書(shū),注意每批的細(xì)節(jié)。在使用細(xì)胞因子前,瞬時(shí)離心試劑瓶,盡可能多地收回其中的細(xì)胞因子。
F9(小鼠畸瘤細(xì)胞)來(lái)源可靠(源于ATCC、ECACC、DSMZ、JCRB等),活力>95%,貼壁性好。我們從試劑、包被器皿、凍存原代細(xì)胞、新鮮原代細(xì)胞、原代細(xì)胞的分子學(xué)實(shí)驗(yàn)等提供全程服務(wù)。我司擁有專業(yè)的實(shí)驗(yàn)室,可無(wú)菌操作并提供相關(guān)科研項(xiàng)目解決方案以及實(shí)驗(yàn)服務(wù)。
服務(wù)流程:
預(yù)約登記→辦理相關(guān)手續(xù)→復(fù)蘇細(xì)胞→細(xì)胞質(zhì)量檢查→發(fā)送細(xì)胞(或自己來(lái)取細(xì)胞)→細(xì)胞售后技術(shù)咨詢答疑。
養(yǎng)方法如下:
1、從手術(shù)室無(wú)菌取腦髓灰質(zhì)或白質(zhì)后,仔細(xì)剝除腦膜、血管和纖維成分,置Hanks液中漂洗一、二次。
2、置于30—50倍體積的Hanks液中,此時(shí)腦組織比較柔軟,反復(fù)吹打即制成細(xì)胞懸液。
3、把懸液注入離心管室溫中直立5—10分鐘后,細(xì)胞或細(xì)胞團(tuán)快自然下沉,脂肪等雜物易漂浮,可吸除上層、反復(fù)二、三次,即可排除脂肪成分和其它碎塊并獲較多細(xì)胞成分。
4、在沉降物中加入適量培養(yǎng)液,通過(guò)紗網(wǎng)成紗布過(guò)濾,計(jì)數(shù)并調(diào)整細(xì)胞密度。
5、接入培養(yǎng)瓶或皿中,置5%CO2溫箱中培養(yǎng)。
適應(yīng)環(huán)境過(guò)程較長(zhǎng),接種后貼壁較慢。貼壁后短期內(nèi)也可能不見(jiàn)細(xì)胞分裂現(xiàn)象,然而一旦生長(zhǎng)后即能迅速進(jìn)入旺盛的增殖狀態(tài)。細(xì)胞傳代可以0.25%胰酶消化處理。
我司其他相關(guān)銷售產(chǎn)品:
Cs-(035)-002747 腎細(xì)胞株/細(xì)胞腺癌細(xì)胞株/細(xì)胞769-P細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002748 腎細(xì)胞株/細(xì)胞腺癌細(xì)胞株/細(xì)胞;ACHN ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002749 腎細(xì)胞株/細(xì)胞腺癌細(xì)胞株/細(xì)胞;769-P ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002750 腎細(xì)胞株/細(xì)胞腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002751 腎細(xì)胞株/細(xì)胞腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002752 腎細(xì)胞株/細(xì)胞腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002753 腎細(xì)胞株/細(xì)胞腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002754 腎系膜細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002755 腎透明細(xì)胞株/細(xì)胞腺癌細(xì)胞株/細(xì)胞786-O[786-0]細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002756 腎透明細(xì)胞株/細(xì)胞腺癌細(xì)胞株/細(xì)胞;786-O [786-0] ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002757 腎透明細(xì)胞株/細(xì)胞腺癌細(xì)胞株/細(xì)胞;769-P ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002758 腎透明細(xì)胞株/細(xì)胞腺癌細(xì)胞株/細(xì)胞,786-O細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002759 腎透明細(xì)胞株/細(xì)胞腺癌細(xì)胞株/細(xì)胞,786-O細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002760 腎透明細(xì)胞株/細(xì)胞腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002761 腎透明細(xì)胞株/細(xì)胞腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002762 腎透明細(xì)胞株/細(xì)胞癌細(xì)胞株/細(xì)胞Caki-2細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002763 腎透明細(xì)胞株/細(xì)胞癌細(xì)胞株/細(xì)胞786-O[786-0]細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002764 腎透明細(xì)胞株/細(xì)胞癌細(xì)胞株/細(xì)胞;LoMet-ccRCC ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002765 腎透明細(xì)胞株/細(xì)胞癌細(xì)胞株/細(xì)胞;HiMet-ccRCC ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002766 腎透明細(xì)胞株/細(xì)胞癌細(xì)胞株/細(xì)胞;Caki-2 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002767 腎透明細(xì)胞株/細(xì)胞癌細(xì)胞株/細(xì)胞;786-O [786-0] ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002768 腎透明細(xì)胞株/細(xì)胞癌皮膚轉(zhuǎn)移細(xì)胞株/細(xì)胞,Caki-1細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002769 腎透明細(xì)胞株/細(xì)胞癌皮膚轉(zhuǎn)移細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002770 腎透明細(xì)胞株/細(xì)胞癌皮膚轉(zhuǎn)移細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002771 腎透明細(xì)胞株/細(xì)胞癌Caki-1細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002772 腎透明細(xì)胞株/細(xì)胞癌;Caki-1 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002773 腎實(shí)質(zhì)細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002774 腎上腺腺瘤細(xì)胞株/細(xì)胞NCI-H205細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002775 腎上腺腺瘤細(xì)胞株/細(xì)胞;NCI-H205 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002776 腎上腺腺瘤細(xì)胞株/細(xì)胞,NCI-H205細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002777 腎上腺神經(jīng)母細(xì)胞株/細(xì)胞瘤細(xì)胞株/細(xì)胞KP-N-NS細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002778 腎上腺神經(jīng)母細(xì)胞株/細(xì)胞瘤細(xì)胞株/細(xì)胞(腦轉(zhuǎn)移);KP-N-NS ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002779 腎上腺神經(jīng)母細(xì)胞株/細(xì)胞瘤細(xì)胞株/細(xì)胞(腦轉(zhuǎn)移),KP-N-NS細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002780 腎上腺神經(jīng)母細(xì)胞株/細(xì)胞瘤細(xì)胞株/細(xì)胞(腦轉(zhuǎn)移) ,規(guī)格: >5×100000cells/瓶
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Cs-(035)-002782 腎上腺皮質(zhì)小細(xì)胞株/細(xì)胞癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002783 腎上腺皮質(zhì)小細(xì)胞株/細(xì)胞癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002784 腎上腺皮質(zhì)腺癌細(xì)胞株/細(xì)胞NCI-H295R細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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Cs-(035)-002786 腎上腺皮質(zhì)腺癌細(xì)胞株/細(xì)胞,SW-13細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002787 腎上腺皮質(zhì)腺癌細(xì)胞株/細(xì)胞,NCI-295R細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002788 腎上腺皮質(zhì)瘤,SW13細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002789 腎上腺皮質(zhì)癌細(xì)胞株/細(xì)胞SW-13細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002790 腎上腺皮質(zhì)癌細(xì)胞株/細(xì)胞;SW-13 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002791 腎上腺癌細(xì)胞株/細(xì)胞ACHN細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002792 腎上腺癌細(xì)胞株/細(xì)胞;ACHN ,規(guī)格: >5×100000cells/瓶
Cs-(035)-002793 腎上皮細(xì)胞株/細(xì)胞,GES細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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